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Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review

Authors
Ren, JunLee, Hyang-MiShen, JunHaoNa, Dokyun
Issue Date
2022
Publisher
SPRINGER
Keywords
Endonucleases; Methylation; Methyltransferase; Restriction-modification system
Citation
BIOTECHNOLOGY LETTERS, v.44, no.1, pp 33 - 44
Pages
12
Journal Title
BIOTECHNOLOGY LETTERS
Volume
44
Number
1
Start Page
33
End Page
44
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52389
DOI
10.1007/s10529-021-03208-9
ISSN
0141-5492
1573-6776
Abstract
Since prokaryotic restriction-modification (RM) systems protect the host by cleaving foreign DNA by restriction endonucleases, it is difficult to introduce engineered plasmid DNAs into newly isolated microorganisms whose RM system is not discovered. The prokaryotes also possess methyltransferases to protect their own DNA from the endonucleases. As those methyltransferases can be utilized to methylate engineered plasmid DNAs before transformation and to enhance the stability within the cells, the study on methyltransferases in newly isolated bacteria is essential for genetic engineering. Here, we introduce the mechanism of the RM system, specifically the methyltransferases and their biotechnological applications. These biotechnological strategies could facilitate plasmid DNA-based genetic engineering in bacteria strains that strongly defend against foreign DNA.
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