In vivo imaging of reactive oxygen species (ROS)-producing pro-inflammatory macrophages in murine carotid atheromas using a CD44-targetable and ROS-responsive nanosensor
- Authors
- Park, E.J.; Song, J.W.; Kim, H.J.; Kim, C.-S.; Song, Y.J.; Yang, D.H.; Yoo, H.; Kim, J.W.; Park, Kyeonoon
- Issue Date
- 25-Dec-2020
- Publisher
- Korean Society of Industrial Engineering Chemistry
- Keywords
- Atherosclerotic plaques; hyaluronan receptors; pro-inflammatory macrophages; reactive oxygen species (ROS) imaging; ROS-sensitive nanosensor
- Citation
- Journal of Industrial and Engineering Chemistry, v.92, pp 158 - 166
- Pages
- 9
- Journal Title
- Journal of Industrial and Engineering Chemistry
- Volume
- 92
- Start Page
- 158
- End Page
- 166
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52862
- DOI
- 10.1016/j.jiec.2020.08.034
- ISSN
- 1226-086X
1876-794X
- Abstract
- In this study, we report the in vivo imaging of reactive oxygen species (ROS)-overproducing pro-inflammatory macrophages in atherosclerotic plaques using a fluorescent ROS nanosensor. We designed the nanosensor by chemically conjugating hyaluronic acid (HA, a targeting ligand for CD44 receptor), chlorin e6 [Ce6; a near-infrared fluorescent (NIRF) dye], and a thioketal (TK) linker (ROS-degradable linker). The self-assembled nanosensor emitted weak NIRF signals in normal physiological conditions, whereas it emitted strong NIRF signals under ROS-abundant conditions. The cytocompatible nanosensor showed higher intracellular internalization via receptor-mediated endocytosis, which enabled the visualization of intracellular ROS in pro-inflammatory macrophages. Moreover, we demonstrated that the nanosensor enabled the successful targeting and imaging of CD44- and ROS-overproducing pro-inflammatory macrophages in atherosclerotic plaques, as validated by confocal microscopy and immunohistological analyses. These data suggest that our ROS nanosensor is suitable for ROS imaging in pro-inflammatory macrophages in vitro and in vivo and will be a promising approach for imaging atherosclerotic tissues and evaluating the effects of antioxidants. © 2020 The Korean Society of Industrial and Engineering Chemistry
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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