Optimization of Extraction Conditions of Continentalic and Kaurenoic Acids from Aralia continentalis by HPLC/UV and Their Validation
- Authors
- Lee, J.S.; Quilantang, N.G.; Hahm, D.-H.; Kang, K.S.; Jacinto, S.D.; Choi, Y.-J.; Lee, S.C.; Lee, S.
- Issue Date
- Aug-2020
- Publisher
- NLM (Medline)
- Citation
- Journal of chromatographic science, v.58, no.7, pp 672 - 677
- Pages
- 6
- Journal Title
- Journal of chromatographic science
- Volume
- 58
- Number
- 7
- Start Page
- 672
- End Page
- 677
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53506
- DOI
- 10.1093/chromsci/bmaa019
- ISSN
- 0021-9665
1945-239X
- Abstract
- Aralia continentalis (AC) is a perennial herb that has long been used as a traditional medicine for many diseases. Continentalic acid (CA) and kaurenoic acid (KA) are major diterpenoids in AC, which are known to exert various pharmacological activities. This study focuses on the optimization of the extraction of CA and KA from dried AC roots by evaluating the influence of different extraction conditions on their yield. Five extraction variables were examined: sample weight, solvent concentration, extraction time, solid matrix and the number of repeated extractions. The analytical method used in this study was also validated in terms of linearity, limit of detection, limit of quantification, precision and accuracy. The CA and KA yields were measured by high-performance liquid chromatography analysis. The results show that CA and KA were the highest when unpulverized samples (3.75 g) were subjected to a single extraction for 5 h using 50% ethanol (300 mL) as the solvent. These conditions are proposed for the optimization of the extraction of CA and KA from AC. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53506)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.