310 nm UV-LEDs attenuate imiquimod-induced psoriasis-like skin lesions in C57BL/6 mice and inhibit IL-22-induced STAT3 expression in HaCaT cells
- Authors
- Kwon, Tae-Rin; Lee, Sung-Eun; Kim, Jong Hwan; Jang, You Na; Kim, Su-Young; Mun, Seok Kyun; Kim, Chan Woong; Na, Jungtae; Kim, Beom Joon
- Issue Date
- Oct-2020
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v.19, no.8, pp 1009 - 1021
- Pages
- 13
- Journal Title
- PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
- Volume
- 19
- Number
- 8
- Start Page
- 1009
- End Page
- 1021
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53530
- DOI
- 10.1039/c9pp00444k
- ISSN
- 1474-905X
1474-9092
- Abstract
- Ultraviolet light-emitting diodes (UV-LEDs) are a novel light source for phototherapy. This study aimed to evaluate the therapeutic effects of UV-LEDs on psoriasis. Importantly, 310 nm UV-LEDs have not been studied in psoriasisin vitroandin vivo. Effects due to 310 nm UV-LED and 311 nm narrowband ultraviolet B (NBUVB) irradiation were compared for suppressing IL-22-induced activation of STAT3 expression using cell viability assay, western blotting, and immunocytochemistry. C57BL/6 mice were topically treated with imiquimod (IMQ) for 6 consecutive days and degenerative changes were observed. Test groups were irradiated with a 310 nm UV-LED and 311 nm NBUVB. Phenotypic observations, histopathological examinations, and ELISA were conducted with skin and blood samples. STAT3-dependent IL-22 signalling and effects in keratinocytes are negatively regulated by the 310 nm UV-LED, which significantly ameliorated IMQ-induced psoriasis-like dermatitis development and reduced Th17 cytokine levels (IL-17A, IL-22) in serum and dorsal skin. Histopathological findings showed decreases in epidermal thickness and inflammatory T-cell infiltration in the UV-LED-irradiated groups. Quantitative PCR confirmed a UV radiation energy-dependent decrease in IL-17A and IL-22 mRNA levels. The results demonstrated that UV-LEDs had anti-inflammatory and immunoregulatory effects. So, UV-LED phototherapy inhibits psoriasis development by suppressing STAT3 protein and inflammatory cytokines and could be useful in treating psoriasis.
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