Comparative Proteomic Analysis for a Putative Pyridoxal Phosphate-Depen- dent Aminotransferase Required for Virulence in Acidovorax citrulliopen access
- Authors
- Lee, Jongchan; Heo, Lynn; Han, Sang-Wook
- Issue Date
- Dec-2021
- Publisher
- 한국식물병리학회
- Keywords
- Acidovorax citrulli; proteomics; pyridoxal phosphate-dependent aminotransferase
- Citation
- The Plant Pathology Journal, v.37, no.6, pp 673 - 680
- Pages
- 8
- Journal Title
- The Plant Pathology Journal
- Volume
- 37
- Number
- 6
- Start Page
- 673
- End Page
- 680
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54503
- DOI
- 10.5423/PPJ.NT.09.2021.0139
- ISSN
- 1598-2254
2093-9280
- Abstract
- Acidovorax citrulli (Ac) is the causative agent of bacte- rial fruit blotch disease in watermelon. Since resistant cultivars have not yet been developed, the virulence factors/mechanisms of Ac need to be characterized. This study reports the functions of a putative pyridoxal phosphate-dependent aminotransferase (PpdaAc) that transfers amino groups to its substrates and uses pyridoxal phosphate as a coenzyme. It was observed that a ppdaAc knockout mutant had a significantly reduced virulence in watermelon when introduced via germinated-seed inoculation as well as leaf infiltration. Comparative proteomic analysis predicted the cellular mechanisms related to PpdaAc. Apart from causing virulence, the PpdaAc may have significant roles in energy production, cell membrane, motility, chemo- taxis, post-translational modifications, and iron-related mechanisms. Therefore, it is postulated that PpdaAc may possess pleiotropic effects. These results provide new insights into the functions of a previously unidenti- fied PpdaAc in Ac.
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