Multifunctional Probiotic and Functional Properties of Lactiplantibacillus plantarum LRCC5314, Isolated from Kimchi
- Authors
- Yoon, Seokmin; Cho, Hyeokjun; Nam, Yohan; Park, Miri; Lim, Ahyoung; Kim, Jong-Hwa; Park, Jaewoong; Kim, Wonyong
- Issue Date
- Jan-2022
- Publisher
- 한국미생물·생명공학회
- Keywords
- Lactiplantibacillus plantarum LRCC5314; probiotics; multifunctional properties; kimchi
- Citation
- Journal of Microbiology and Biotechnology, v.32, no.1, pp 72 - 80
- Pages
- 9
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 32
- Number
- 1
- Start Page
- 72
- End Page
- 80
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54828
- DOI
- 10.4014/jmb.2109.09025
- ISSN
- 1017-7825
1738-8872
- Abstract
- In this study, the survival capacity (acid and bile salt tolerance, and adhesion to gut epithelial cells) and probiotic properties (enzyme activity-inhibition and anti-inflammatory activities, inhibition of adipogenesis, and stress hormone level reduction) of Lactiplantibacillus plantarum LRCC5314, isolated from kimchi (Korean traditional fermented cabbage), were investigated. LRCC5314 exhibited very stable survival at ph 2.0 and in 0.2% bile acid with 89.9% adhesion to Caco-2 intestinal epithelial cells after treatment for 2 h. LRCC5314 also inhibited the activities of α-amylase and α-glucosidase, which are involved in elevating postprandial blood glucose levels, by approximately 72.9% and 51.2%, respectively. Treatment of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with the LRCC5314 lysate decreased the levels of the inflammatory factors nitric oxide, tumor necrosis factor (TNF-α), interleukin (IL)-1β, and interferon-γ by 88.5%, 49.3%, 97.2%, and 99.8%, respectively, relative to those of the cells treated with LPS alone. LRCC5314 also inhibited adipogenesis in differentiating preadipocytes (3T3-L1 cells), showing a 14.7% decrease in lipid droplet levels and a 74.0% decrease in triglyceride levels, as well as distinct reductions in the mRNA expression levels of adiponectin, FAS, PPAR/γ, C/EBPα, TNF-α, and IL-6. Moreover, LRCC5314 reduced the level of cortisol, a hormone with important effect on stress, by approximately 35.6% in H295R cells. L. plantarum LRCC5314 is identified as a new probiotic with excellent in vitro multifunctional properties. Subsequent in vivo studies may further demonstrate its potential as a functional food or pharmabiotic.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.