Suppression of CFTR-mediated Cl- Secretion of Airway Epithelium in Vitamin C-deficient Mice
- Authors
- Kim, Yeryung; Kim, Hyemin; Yoo, Hae-Young; Kang, Jae Seung; Kim, Sung Joon; Kim, Jin Kyoung; Cho, Hyun Sung
- Issue Date
- Mar-2011
- Publisher
- KOREAN ACAD MEDICAL SCIENCES
- Keywords
- Hyperoxia; Airway Epithelium; Cystic Fibrosis Transmembrane Conductance Regulator; Electrolyte Transport; Ascorbic Acid
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, v.26, no.3, pp 317 - 324
- Pages
- 8
- Journal Title
- JOURNAL OF KOREAN MEDICAL SCIENCE
- Volume
- 26
- Number
- 3
- Start Page
- 317
- End Page
- 324
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55292
- DOI
- 10.3346/jkms.2011.26.3.317
- ISSN
- 1011-8934
1598-6357
- Abstract
- Hyperoxic ventilation induces detrimental effects on the respiratory system, and ambient oxygen may be harmful unless compensated by physiological anti-oxidants, such as vitamin C. Here we investigate the changes in electrolyte transport of airway epithelium in mice exposed to normobaric hyperoxia and in gulonolacton oxidase knock-out (gulo[-/-]) mice without vitamin C (Vit-C) supplementation. Short-circuit current (l(sc)) of tracheal epithelium was measured using Ussing chamber technique. After confirming amiloride-sensitive Na+ absorption (Delta l(sc,amil)), cAMP-dependent Cl- secretion (Delta l(sc,Forsk)) was induced by forskolin. To evaluate Ca2+-dependent Cl- secretion, ATP was applied to the luminal side (Delta l(sc),(ATP)). In mice exposed to 98% PO2 for 36 hr, Delta l(sc,forsk) decreased, Delta l(sc,amil) and Delta l(sc,ATP) was not affected. In gulo(-/-) mice, both Delta l(sc,forsk) and Delta l(sc,ATP) decreased from three weeks after Vit-C deprivation, while both were unchanged with Vit-C supplementation. At the fourth week, tissue resistance and all electrolyte transport activities were dezreased. An immunofluorescence study showed that the expression of cystic fibrosis conductance regulator (CFTR) was decreased in gulo(-/-) mice, whereas the expression of KCNQ1 K+ channel was preserved. Taken together, the CFTR-mediated Cl- secretion of airway epithelium is susceptible to oxidative stress, which suggests that supplementation of the antioxidant might be beneficial for the maintenance of airway surface liquid.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Red Cross College of Nursing > Department of Nursing > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55292)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.