Customized Multilayered Tissue-on-a-Chip (MToC) to Simulate Bacillus Calmette–Guérin (BCG) Immunotherapy for Bladder Cancer Treatment
- Authors
- Lee, S.; Kim, J.H.; Kang, S.J.; Chang, In Ho; Park, Joong Yull
- Issue Date
- Mar-2022
- Publisher
- SpringerOpen
- Keywords
- 3D bioprinting; Bacillus Calmette–Guérin (BCG); Bladder cancer; Cancer-on-a-chip; Immunotherapy; Non-muscle invasive bladder cancer (NMIBC)
- Citation
- Biochip Journal, v.16, no.1, pp 67 - 81
- Pages
- 15
- Journal Title
- Biochip Journal
- Volume
- 16
- Number
- 1
- Start Page
- 67
- End Page
- 81
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55355
- DOI
- 10.1007/s13206-022-00047-2
- ISSN
- 1976-0280
2092-7843
- Abstract
- As an advanced cell culture platform, the organ-on-a-chip has been in the spotlight recently owing to its ability to maintain the physiological characteristics of cells in vitro. Therefore, several disease models have been developed using the organ-on-a-chip technology, and the organ specific three-dimensional (3D) structure and various mechanical/chemical stimuli built into the chip enable efficient development of drugs, medical devices, and biomaterials, as well as realization of patient-specific precise medicine. This study introduces a novel chip-based non-muscle invasive bladder cancer model, multilayered tissue-on-a-chip (MToC), which was created using 3D bio-printing technology, micro-milling, and soft lithography based polydimethylsiloxane (PDMS) casting. All types of cells, T24, MRC-5, and HUVEC, were successfully co-cultured in the MToC. Using computational fluid dynamics (CFD), the flow phenomena occurring in MToC were analyzed. Further, we attempted Bacillus Calmette–Guérin (BCG)-induced migration of THP-1, and the viability reduction of bladder cancer cells and the THP-1 migration were observed. Although follow-up studies are needed to precisely mimic the immune response, this partial phenomenon of the immune response suggests the potential of this device as a surrogate experimental tool for BCG immunotherapy in future. © 2022, The Korean BioChip Society.
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