Urinary hsv2-miR-H9 to hsa-miR-3659 ratio is an effective marker for discriminating prostate cancer from benign prostate hyperplasia in patients within the prostate-specific antigen grey zoneopen access
- Authors
- 강호원; 변영준; 문성민; 김경; Piao, X.-M.; Zheng, C.-M.; 문성권; 최영현; 김원태; 김용준; 이상철; 윤석중; 김원재
- Issue Date
- Mar-2022
- Publisher
- 대한비뇨의학회
- Keywords
- Biomarker; Diagnosis; Microarray analysis; MicroRNAs; Prostatic neoplasms
- Citation
- Investigative and Clinical Urology, v.63, no.2, pp 238 - 244
- Pages
- 7
- Journal Title
- Investigative and Clinical Urology
- Volume
- 63
- Number
- 2
- Start Page
- 238
- End Page
- 244
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55801
- DOI
- 10.4111/icu.20210493
- ISSN
- 2466-0493
2466-054X
- Abstract
- Purpose: Tumor microRNAs (miRNAs) are released to biofluids directly or indirectly. Although urinary miRNAs are promising non-invasive biomarkers for the diagnosis of prostate cancer (PCa), their clinical application is challenging for technical reasons. We examined the efficacy of urinary hsv2-miR-H9 to hsa-miR-3659 ratio as a non-invasive diagnostic biomarker of PCa.
Materials and Methods: The expression of urinary miRNAs was quantified by real-time PCR in 116 samples from 53 patients with benign prostatic hyperplasia (BPH) and 63 patients with PCa. The miRNA expression ratio was calculated using an upregulated miRNA (hsv2-miR-H9) as the numerator and a downregulated miRNA (hsa-miR-3659) as the denominator.
Results: The urinary miR-H9 to miR-3659 ratio was significantly higher in PCa than in BPH controls (p<0.001). The diagnostic accuracy of the urinary miRNA expression ratio was comparable with that of prostate-specific antigen (PSA) (receiver operating characteristic [ROC] curve comparison, p=0.287). The area under the curve for urinary miRNA expression ratio was 0.862 and that for PSA was 0.642 in the “PSA gray zone” (3–10 ng/mL) (ROC curve comparison, p=0.034). The use of the urinary miRNA expression ratio would have prevented 70.6% of unnecessary prostate biopsies; however, 28.6% of PCa cases could be missed in patients within the PSA gray zone.
Conclusions: The expression ratio of urinary miR-H9 to miR-3659 could be a relevant non-invasive biomarker for PCa diagnosis, particularly for patients within the PSA gray zone.
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