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A microscopic investigation on the effect of hydrophobic properties on cell adhesion on a PDMS surface
- Authors
- Chung, Sung Hee; Min, Junhong
- Issue Date
- Jun-2008
- Publisher
- KOREAN BIOCHIP SOCIETY-KBCS
- Keywords
- HMEC; MCF-7; hydrophobicity; PDMS; adhesion
- Citation
- BIOCHIP JOURNAL, v.2, no.2, pp 141 - 147
- Pages
- 7
- Journal Title
- BIOCHIP JOURNAL
- Volume
- 2
- Number
- 2
- Start Page
- 141
- End Page
- 147
- ISSN
- 1976-0280
2092-7843
- Abstract
- In vitro cultivations of human mammary epithelial cells (HMEC), a human breast cancer cell line (MCF-7), and human brain cancer cells (U373MG) were performed in a PDMS chamber without any cell adhesion receptor or surface coating. The surfaces were treated by the exposure of air plasma to control the hydrophobic properties. The effects of the hydrophobic properties of PDMS on cell adhesion and growth were microscopically investigated, and their viability measured using a live and dead cell kit by laser confocal microscopy. The adhesion force difference between cells (HMEC and MCF-7) was investigated using shear stress induced by a microflow in the micro channel.
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