Genetic Mapping of Resistant Genes in Brassica pekinensis Against Plasmodiophora brassicae Race 6
- Authors
- Lee, Gung Pyo; Baek, Nam Kwon; Park, Kuen Woo
- Issue Date
- 2002
- Publisher
- 한국식물병리학회
- Keywords
- Brassica pekinensis; clubroot; genetic mapping; Plasmodiophora brassicae; QTL.
- Citation
- The Plant Pathology Journal, v.18, no.5, pp 266 - 270
- Pages
- 5
- Journal Title
- The Plant Pathology Journal
- Volume
- 18
- Number
- 5
- Start Page
- 266
- End Page
- 270
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56600
- ISSN
- 1598-2254
2093-9280
- Abstract
- Inbred lines of Chinese cabbage KU-101 (resistant line
against Plasmodiophora brassicae race 6) and CS-113
(susceptible line) were crossed and their progeny lines
F1, BC1F1, F2, and F3 were produced for the construction
of the genetic linkage map of P. brassicae race 6-
resistant Brassica campestris ssp. pekinensis genome.
Restriction fragment length polymorphism (RFLP) was
applied to compare between parents and their F2
progenies with a total of 192 probes and 5 restriction
enzymes. The constructed RFLP map covered 1,104 cM
with a mean distance between genetic marker of 8.0 cM,
and produced 10 linkage groups having 121 genetic loci.
The loci of P. brassicae race 6 (CR6)-resistant Brassica
genome were determined by interval mapping of quantitative
trait loci (QTL), which resulted from bioassay
using the same race of the fungi in F3 population.
Resistant loci were estimated in numbers 1 (G1) and 3
(G3) linkage groups. In the regression test, G1 had a
value of 4.8 logarithm of odd (LOD) score, while G3 had
values of 4.2-7.2. Given these results, the location of the
CR6-resistant loci within the Brassica genome map can
now be addressed.
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