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GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorphaopen access
- Authors
- Ubiyvovk, Vira M.; Nazarko, Taras Y.; Stasyk, Olena G.; Sohn, Min Jeong; Kang, Hyun Ah; Sibirny, Andrei A.
- Issue Date
- Aug-2002
- Publisher
- OXFORD UNIV PRESS
- Keywords
- γ-Glutamylcysteine synthetase; Glutathione biosynthesis; Hansenula polymorpha; Methylotrophic yeast
- Citation
- FEMS YEAST RESEARCH, v.2, no.3, pp 327 - 332
- Pages
- 6
- Journal Title
- FEMS YEAST RESEARCH
- Volume
- 2
- Number
- 3
- Start Page
- 327
- End Page
- 332
- ISSN
- 1567-1356
1567-1364
- Abstract
- The GSH2 gene, encoding Hansenula polymorpha, gamma-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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