Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, M.-D. | - |
dc.contributor.author | Kang, H.-A. | - |
dc.contributor.author | Rhee, S.-K. | - |
dc.contributor.author | Seo, J.-H. | - |
dc.date.accessioned | 2022-04-29T07:40:13Z | - |
dc.date.available | 2022-04-29T07:40:13Z | - |
dc.date.issued | 2001-07 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.issn | 1476-5535 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56999 | - |
dc.description.abstract | A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration of 1.7 g l(-1) was determined ata dilution rate of 0.18 h(-1) with 1.8 mg l(-1) h(-1) hirudin productivity. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE AMERICA INC | - |
dc.title | Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/sj.jim.7000163 | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.27, no.1, pp 58 - 61 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000171055800010 | - |
dc.identifier.scopusid | 2-s2.0-0034786086 | - |
dc.citation.endPage | 61 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 58 | - |
dc.citation.title | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.citation.volume | 27 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Hansenula polymorpha | - |
dc.subject.keywordAuthor | Hirudin | - |
dc.subject.keywordAuthor | Methanol | - |
dc.subject.keywordAuthor | Methanol oxidase promoter | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | METHYLOTROPHIC YEASTS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordPlus | CULTURES | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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