Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha
- Authors
- Kim, M.-D.; Kang, H.-A.; Rhee, S.-K.; Seo, J.-H.
- Issue Date
- Jul-2001
- Publisher
- NATURE AMERICA INC
- Keywords
- Hansenula polymorpha; Hirudin; Methanol; Methanol oxidase promoter
- Citation
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.27, no.1, pp 58 - 61
- Pages
- 4
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- Volume
- 27
- Number
- 1
- Start Page
- 58
- End Page
- 61
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56999
- DOI
- 10.1038/sj.jim.7000163
- ISSN
- 1367-5435
1476-5535
- Abstract
- A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration of 1.7 g l(-1) was determined ata dilution rate of 0.18 h(-1) with 1.8 mg l(-1) h(-1) hirudin productivity.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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