Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 geneopen access
- Authors
- Kang, Hyun Ah; Kim, Jeong-Yoon; Ko, Su-Min; Park, Cheon Seok; Ryu, Dewey D.Y.; Sohn, Jung-Hoon; Choi, Eui-Sung; Rhee, Sang-Ki
- Issue Date
- Sep-1998
- Publisher
- JOHN WILEY & SONS LTD
- Keywords
- Ca2+-ATPase; Hansenula polymorpha; PMR1; Yeast
- Citation
- YEAST, v.14, no.13, pp 1233 - 1240
- Pages
- 8
- Journal Title
- YEAST
- Volume
- 14
- Number
- 13
- Start Page
- 1233
- End Page
- 1240
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57026
- DOI
- 10.1002/(SICI)1097-0061(19980930)14:13<1233::AID-YEA322>3.0.CO;2-Y
- ISSN
- 0749-503X
1097-0061
- Abstract
- A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPases. The cloned gene product exhibits 60.3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H, polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P-type Ca2+-ATPase. The DNA sequence of the H, polymorpha homologue has been submitted to GenBank with the Accession Number U92083. (C) 1998 John Wiley & Sons, Ltd.
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