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지렁이 중장에서 분리한 alkaline phosphatase의 분포와 특성에 관한 연구Distribution and Some Properties of the Alkaline Phosphatase from the Midgut of the Earthworm, Eisenia andrei
- Issue Date
- Jun-2000
- Publisher
- 한국토양동물학회
- Keywords
- Alkaline phosphatase; Earthworm; Midgut; Distribution; Characteristics
- Citation
- 한국토양동물학회지, v.5, no.1, pp 39 - 46
- Pages
- 8
- Journal Title
- 한국토양동물학회지
- Volume
- 5
- Number
- 1
- Start Page
- 39
- End Page
- 46
- Abstract
- The distribution and some properties of alkaline phosphatase (ALP) were investigated in the midgut of the earthworm, Eisenia andrei. The ALP activity appeared to be highly polarized toward the luminal side of epithelium, with minor ALP activity in chloragogue tissue. The epithelial and chloragogeneous tissues contained approximately 85 and 15% of total intestinal ALP activity, respectively. The optimal temperature was approximately 37$^{circ}C$ and isoelectricpoint was estimated to be 4. The treatment of neuraminidase and PtdIns-PLC failed to change the migration rate of ALPs. Also, these ALPs appeared to have a wide range of substrate specificity. The relationship between the properties and physiological significances of the midgut ALPs in Eisenia andrei was discussed.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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