Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ramisetti, Srinivas | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.contributor.author | Rhee, Sang Ki | - |
dc.contributor.author | Kim, Chul Ho | - |
dc.date.accessioned | 2022-05-11T02:40:35Z | - |
dc.date.available | 2022-05-11T02:40:35Z | - |
dc.date.issued | 2003-06 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.issn | 1976-3816 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57522 | - |
dc.description.abstract | The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L. ©KSBB. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society for Biotechnology and Bioengineering | - |
dc.title | Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/BF02935894 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, v.8, no.3, pp 183 - 186 | - |
dc.identifier.kciid | ART001211634 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000188831200004 | - |
dc.identifier.scopusid | 2-s2.0-7544244720 | - |
dc.citation.endPage | 186 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 183 | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.volume | 8 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Fed-batch fermentation | - |
dc.subject.keywordAuthor | GAL promoter | - |
dc.subject.keywordAuthor | Galactose | - |
dc.subject.keywordAuthor | Recombinant hirudin | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | ANTICOAGULANT HIRUDINOPTIMIZATIONAGENT | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kciCandi | - |
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