Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding
- Authors
- Ramisetti, Srinivas; Kang, Hyun Ah; Rhee, Sang Ki; Kim, Chul Ho
- Issue Date
- Jun-2003
- Publisher
- Korean Society for Biotechnology and Bioengineering
- Keywords
- Fed-batch fermentation; GAL promoter; Galactose; Recombinant hirudin; Saccharomyces cerevisiae
- Citation
- Biotechnology and Bioprocess Engineering, v.8, no.3, pp 183 - 186
- Pages
- 4
- Journal Title
- Biotechnology and Bioprocess Engineering
- Volume
- 8
- Number
- 3
- Start Page
- 183
- End Page
- 186
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57522
- DOI
- 10.1007/BF02935894
- ISSN
- 1226-8372
1976-3816
- Abstract
- The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L. ©KSBB.
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