NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells
- Authors
- Das, Sudipto; Hahn, Yoonsoo; Nagata, Satoshi; Willingham, Mark C.; Bera, Tapan K.; Lee, Byungkook; Pastan, Ira
- Issue Date
- Feb-2007
- Publisher
- AMER ASSOC CANCER RESEARCH
- Citation
- CANCER RESEARCH, v.67, no.4, pp 1594 - 1601
- Pages
- 8
- Journal Title
- CANCER RESEARCH
- Volume
- 67
- Number
- 4
- Start Page
- 1594
- End Page
- 1601
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57571
- DOI
- 10.1158/0008-5472.CAN-06-2673
- ISSN
- 0008-5472
- Abstract
- NGEP is a prostate-specific gene identified by analysis of expressed sequence tag databases. RNA analysis revealed two spliced forms of NGEP mRNA: a short form encoding a soluble protein (NGEP-S) and a long form encoding a polytopic membrane protein (NGEP-L). Transient expression of myc epitope-tagged NGEP-L showed that it was localized to the plasma membrane. We have now produced a specific antibody to the COOH terminus of NGEP-L and showed that it detects an similar to 100-kDa protein in extracts of normal prostate and prostate cancers that contain high levels of NGEP mRNA. The antibody detects a protein that is highly expressed on the apical and the lateral surfaces of normal prostate and prostate cancer cells by immunohistochemistry. The antibody does not detect a protein in the prostate cancer cell line LNCaP, which has very low NGEP mRNA levels. To study NGEP function, two stable LNCaP cell lines were prepared by transfection with NGEP-L and shown to contain similar amounts of NGEP-L protein as human prostate. Confocal immunofluorescence showed that NGEP-L is present on the plasma membrane of the transfected LNCaP cells and is highly concentrated at cell:cell contact regions. Furthermore, as the cell density increased, the cells formed large aggregates. A specific RNA interference that lowered NGEP-L levels prevented formation of cell aggregates. Our results suggest that NGEP-L has a role in promoting cell contact-dependent interactions of LNCaP prostate cancer cells and also that NGEP is a promising immunotherapy target for prostate cancer.
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