Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags
- Authors
- Jung, J.D.; Park, H.-W.; Hahn, Yoonsoo; Hur, C.-G.; In, D.S.; Chung, H.-J.; Liu, J.R.; Choi, D.-W.
- Issue Date
- Aug-2003
- Publisher
- SPRINGER
- Keywords
- expressed sequence tags; ginsenoside; Panax ginseng; oxidosqualene cyclase; secondary metabolism
- Citation
- PLANT CELL REPORTS, v.22, no.3, pp 224 - 230
- Pages
- 7
- Journal Title
- PLANT CELL REPORTS
- Volume
- 22
- Number
- 3
- Start Page
- 224
- End Page
- 230
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57589
- DOI
- 10.1007/s00299-003-0678-6
- ISSN
- 0721-7714
1432-203X
- Abstract
- Expressed sequence tags (ESTs) provide a valuable tool that can be used to identify genes in secondary metabolite biosynthesis. Ginseng (Panax ginseng C.A Meyer) is a medicinal plant that accumulates ginsenosides in roots. We sequenced 11,636 ESTs from five ginseng libraries in order to create a gene resource for biosynthesis of ginsenosides, which are thought to be the major active component in roots. Only 59% of the ginseng ESTs exhibited significant homology to previously known polypeptide sequences. Stress- and pathogen-response proteins were most abundant in 4-year-old ginseng roots. ESTs involved in ginsenoside biosynthesis were identified by a keyword search of BLASTX results and a domain search of ginseng ESTs. We identified 4 oxidosqualene cyclase candidates involved in the cyclization reaction of 2,3-oxidosqualene, 9 nine cytochrome P450 and 12 glycosyltransferse candidates, which may be involved in modification of the triterpene backbone.
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