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Cloning of mouse sepiapterin reductase gene and characterization of its promoter region
- Authors
- Lee, Soo Woong; Park, In Young; Hahn, Yoonsoo; Lee, Ji Eun; Seong, Chang Soo; Chung, Jae Hoon; Park, Young Shik
- Issue Date
- Apr-1999
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- tetrahydrobiopterin; promoter; luciferase assay; pseudogene
- Citation
- BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, v.1445, no.1, pp 165 - 171
- Pages
- 7
- Journal Title
- BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
- Volume
- 1445
- Number
- 1
- Start Page
- 165
- End Page
- 171
- ISSN
- 0167-4781
- Abstract
- We have isolated and characterized similar to 5 kb mouse sepiapterin reductase gene (Spr) and a highly homologous pseudogene (Sprp). The authentic Spr gene is present as a single copy in the mouse genome and is composed of three exons containing the entire coding region. The primer extension experiment located the transcription initiation site in a putative pyrimidine-rich Inr element. The promoter region of the Spr gene is embedded within a CpG island. It was shown that the promoter region is devoid of distinctive TATA and CAAT boxes. Transient transfection of a series of 5' deletion derivatives of the Spr promoter showed the sequence between -83 and -51 to be essential for promoter activity. The pseudogene Sprp lacks promoter region and exon 3. (C) 1999 Elsevier Science B.V. All rights reserved.
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