Fringe forms a complex with Notch
- Authors
- Ju, Bong-Gun; Jeong, Sangyun; Bae, Eunkyung; Hyun, Seogang; Carroll, Sean B.; Yim, Jeongbin; Kim, Jaeseob
- Issue Date
- May-2000
- Publisher
- MACMILLAN MAGAZINES LTD
- Citation
- NATURE, v.405, no.6783, pp 191 - 195
- Pages
- 5
- Journal Title
- NATURE
- Volume
- 405
- Number
- 6783
- Start Page
- 191
- End Page
- 195
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57618
- DOI
- 10.1038/35012090
- ISSN
- 0028-0836
1476-4687
- Abstract
- The Fringe protein of Drosophila and its vertebrate homologues function in boundary determination during pattern formation(1-9). Fringe has been proposed to inhibit Serrate-Notch signalling but to potentiate Delta-Notch signalling(10). Here we show that Fringe and Notch form a complex through both the Lin-Notch repeats and the epidermal growth factor repeats 22-36 (EGF22-36) of Notch when they are co-expressed. The Abruptex(59b) (Ax(59b)) and Ax(M1) mutations, which are caused by missense mutations in EGF repeats 24 and 25, respectively, abolish the Fringe-Notch interaction through EGF22-36, whereas the l(1)N-B mutation in the third Lin-Notch repeat of Notch abolishes the interaction through Lin-Notch repeats. Ax mutations also greatly affect the Notch response to ectopic Fringe in vivo. Results from in vitro protein mixing experiments and subcellular colocalization experiments indicate that the Fringe-Notch complex may form before their secretion. These findings explain how Fringe acts cell-autonomously to modulate the ligand preference of Notch and why the Fringe-Notch relationship is conserved between phyla and in the development of very diverse structures.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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