Graphene Oxide-Mediated Fluorometric Aptasensor for Okadaic Acid Detection
- Authors
- Kweon, So Yeon; Park, Jong Pil; Park, Chan Yeong; Park, Tae Jung
- Issue Date
- Jun-2022
- Publisher
- KOREAN BIOCHIP SOCIETY-KBCS
- Keywords
- Marine toxin; Okadaic acid; Graphene oxide; Fluorescence; Aptasensor
- Citation
- BIOCHIP JOURNAL, v.16, no.2, pp 207 - 213
- Pages
- 7
- Journal Title
- BIOCHIP JOURNAL
- Volume
- 16
- Number
- 2
- Start Page
- 207
- End Page
- 213
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57818
- DOI
- 10.1007/s13206-022-00056-1
- ISSN
- 1976-0280
2092-7843
- Abstract
- Okadaic acid (OA), which is one of the representative diarrhetic shellfish poisonings, is a prevalent food-poisoning toxin in the world. As OA consumption induces various digestive diseases, a strict monitoring tool for OA is highly required. In this study, we demonstrate the development of an aptamer-based fluorometric OA sensor. First, the affinity of the aptamer towards OA was confirmed through electrochemical impedance spectroscopy, and it was then utilized to develop the fluorometric sensor. The verified OA-specific aptamer was modified with 5-carboxyfluorescein (FAM) as a fluorophore, and graphene oxide (GO) was adopted as the quencher. By virtue of the quenching phenomena between FAM and GO, the developed sensor revealed a low detection limit of 6.35 ppb. Also, this sensor represented a high response to OA compared to other types of marine toxins, such as saxitoxin, domoic acid, and tetrodotoxin.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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