Elimination of biosynthetic pathways for L-valine and L-isoleucine in mitochondria enhances isobutanol production in engineered Saccharomyces cerevisiae
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyung-Muk | - |
dc.contributor.author | Kim, Sun-Ki | - |
dc.contributor.author | Lee, Ye-Gi | - |
dc.contributor.author | Park, Kyung-Hye | - |
dc.contributor.author | Seo, Jin-Ho | - |
dc.date.available | 2019-01-22T12:29:38Z | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.issn | 1873-2976 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/579 | - |
dc.description.abstract | Saccharomyces cerevisiae has a natural ability to produce higher alcohols, making it a promising candidate for production of isobutanol. However, the several pathways competing with isobutanol biosynthesis lead to production of substantial amounts of L-valine and L-isoleucine in mitochondria and isobutyrate, L-leucine, and ethanol in cytosol. To increase flux to isobutanol by removing by-product formation, the genes associated with formation of L-valine (BATA L-isoleucine (ILV1), isobutyrate (ALD6), L-leucine (LEU1), and ethanol (ADH1) were disrupted to construct the S. cerevisiae W Delta GBIALA1_2vec strain. This strain showed 8.9 and 8.6 folds increases in isobutanol concentration and yield, respectively, relative the corresponding values of the background strain on glucose medium. In a bioreactor fermentation with a gas trapping system, the W Delta GBIALA1_2vec strain produced 662 mg/L isobutanol concentration with a yield of 6.71 mg(i)(sobutanol)/g(glucose). With elimination of the competing pathways, the W Delta GBIALA1_2vec strain would serve as a platform strain for isobutanol production. | - |
dc.format.extent | 7 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Elimination of biosynthetic pathways for L-valine and L-isoleucine in mitochondria enhances isobutanol production in engineered Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.biortech.2018.07.150 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.268, pp 271 - 277 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000445043600034 | - |
dc.identifier.scopusid | 2-s2.0-85050858368 | - |
dc.citation.endPage | 277 | - |
dc.citation.startPage | 271 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 268 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Isobutanol | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Gas trapping | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | CHAIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ALCOHOLS | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | IMPROVEMENT | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | BIOFUELS | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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