Elimination of biosynthetic pathways for L-valine and L-isoleucine in mitochondria enhances isobutanol production in engineered Saccharomyces cerevisiae
- Authors
- Lee, Kyung-Muk; Kim, Sun-Ki; Lee, Ye-Gi; Park, Kyung-Hye; Seo, Jin-Ho
- Issue Date
- Nov-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Isobutanol; Saccharomyces cerevisiae; Metabolic engineering; Gas trapping
- Citation
- BIORESOURCE TECHNOLOGY, v.268, pp 271 - 277
- Pages
- 7
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 268
- Start Page
- 271
- End Page
- 277
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/579
- DOI
- 10.1016/j.biortech.2018.07.150
- ISSN
- 0960-8524
1873-2976
- Abstract
- Saccharomyces cerevisiae has a natural ability to produce higher alcohols, making it a promising candidate for production of isobutanol. However, the several pathways competing with isobutanol biosynthesis lead to production of substantial amounts of L-valine and L-isoleucine in mitochondria and isobutyrate, L-leucine, and ethanol in cytosol. To increase flux to isobutanol by removing by-product formation, the genes associated with formation of L-valine (BATA L-isoleucine (ILV1), isobutyrate (ALD6), L-leucine (LEU1), and ethanol (ADH1) were disrupted to construct the S. cerevisiae W Delta GBIALA1_2vec strain. This strain showed 8.9 and 8.6 folds increases in isobutanol concentration and yield, respectively, relative the corresponding values of the background strain on glucose medium. In a bioreactor fermentation with a gas trapping system, the W Delta GBIALA1_2vec strain produced 662 mg/L isobutanol concentration with a yield of 6.71 mg(i)(sobutanol)/g(glucose). With elimination of the competing pathways, the W Delta GBIALA1_2vec strain would serve as a platform strain for isobutanol production.
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