Aspergillus niger 글루코오스 산화효소의 유도 발현

Abstract

Glucose oxidase catalyzes the oxidation of β -D-glucose to gluconic acid. It is necessary to be obtained maximum glucose oxidase activity because the enzyme is used commercially for various applications, in particular extracelluar glucose oxidase. For high activity of extracellular glucose oxidase, we used the enzyme from Aspergillus niger and YEP medium. Extracellular glucose oxidase activity as high as 7.5Uml^(-1) was obtained with sucrose as carbon source and peptone as nitrogen source. The result of testing a various of medium condition was obtainable the highest enzyme activity in condition of culture medium of 1% sucrose(w/v), 2% peptone(w/v) and 1% yeast extract(w/v) at 25℃. Our work indicate an economically attractive process for enzyme production. In addition Aspergillus niger glucose oxidase was purified by DEAE-Sephacel chromatography. The activity of the enzyme proceeded at pH 5.5 and the effective substrate of the enzyme was only glucose.