3D Multicellular Tumor Spheroids in a Microfluidic Droplet System for Investigation of Drug Resistanceopen access
- Authors
- Lee, Sang Ik; Choi, Yoon Young; Kang, Seong Goo; Kim, Tae Hyeon; Choi, Ji Wook; Kim, Young Jae; Kim, Tae-Hyung; Kang, Taewook; Chung, Bong Geun
- Issue Date
- Sep-2022
- Publisher
- MDPI
- Keywords
- microdroplet; concentration gradient generator; multicellular cancer spheroid; anti-cancer drug screening
- Citation
- POLYMERS, v.14, no.18
- Journal Title
- POLYMERS
- Volume
- 14
- Number
- 18
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58958
- DOI
- 10.3390/polym14183752
- ISSN
- 2073-4360
2073-4360
- Abstract
- A three-dimensional (3D) tumor spheroid model plays a critical role in mimicking tumor microenvironments in vivo. However, the conventional culture methods lack the ability to manipulate the 3D tumor spheroids in a homogeneous manner. To address this limitation, we developed a microfluidic-based droplet system for drug screening applications. We used a tree-shaped gradient generator to control the cell density and encapsulate the cells within uniform-sized droplets to generate a 3D gradient-sized tumor spheroid. Using this microfluidic-based droplet system, we demonstrated the high-throughput generation of uniform 3D tumor spheroids containing various cellular ratios for the analysis of the anti-cancer drug cytotoxicity. Consequently, this microfluidic-based gradient droplet generator could be a potentially powerful tool for anti-cancer drug screening applications.
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Collections - College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
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