Implication of two glutathione S-transferases in the optimal metabolism of m-toluate by Sphingomonas yanoikuyae B1
- Authors
- Bae, Mihyun.; Sul, Woo Jun; Koh,Sung-Cheol; Lee, Joon H.; Zylstra, Gerben J.; Kim,Young Min; Kim, Eungbin
- Issue Date
- Jul-2003
- Keywords
- Aromatic hydrocarbon; Glutathione; m-toluate; S-transferase; Sphingomonas
- Citation
- Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, v.84, no.1, pp 25 - 30
- Pages
- 6
- Journal Title
- Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
- Volume
- 84
- Number
- 1
- Start Page
- 25
- End Page
- 30
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58975
- DOI
- 10.1023/A:1024428715390
- ISSN
- 0003-6072
1572-9699
- Abstract
- A putative glutathione S-transferase (GST) gene (bphK) was identified in the meta-cleavage operon for the degradation of m-toluate by Sphingomonas yanoikuyae B1. Disruption of bphK resulted in the loss of GST activity against 1-chloro-2,4-dinitrobenzene and a much increased lag time of the mutant strain MB3 (bphK::Km) following subculture into m-toluate medium. In contrast, an increased lag time was not observed when MB3 was grown on biphenyl or m-xylene and MB3 showed normal growth on m-toluate when complemented with a subclone containing the bphK gene only. Furthermore, an additional GST activity was detected in MB3. The induction timing of this second GST activity coincided with the beginning of the exponential growth phase of MB3 on m-toluate, reached maximal activity within three hours, and then dropped sharply to the basal level. Thus, it is apparent that BphK and/or the second GST are necessary for optimal growth of B1 on m-toluate.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58975)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.