Identification of a gene cluster for D-tagatose utilization in Escherichia coli B2 phylogroupopen access
- Authors
- Ha, Jinyoung; Kim, Dohyeon; Yeom, Jinho; Kim, Youngshin; Yoo, Seung Min; Yoon, Sung Ho
- Issue Date
- Dec-2022
- Publisher
- Elsevier Inc.
- Keywords
- Bacteriology; Genetics
- Citation
- iScience, v.25, no.12
- Journal Title
- iScience
- Volume
- 25
- Number
- 12
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/59956
- DOI
- 10.1016/j.isci.2022.105655
- ISSN
- 2589-0042
2589-0042
- Abstract
- D-Tagatose is a promising low-calorie sugar-substituting sweetener in the food industry. Most ingested D-tagatose is fermented by intestinal microorganisms. Until now, Escherichia coli has been considered incapable of growing on D-tagatose. Here, we discovered a gene cluster involved in D-tagatose utilization in E. coli. The chromosome of the intestinal probiotic E. coli Nissle 1917 contains a six-gene cluster encoding the ABC transporter, D-tagatose kinase, D-tagatose-bisphosphate aldolase, and putative aldose 1-epimerase. The functionality of the gene cluster was experimentally validated. Based on single-gene deletions, D-tagatose dissimilation occurs via D-tagatose 6-phosphate to D-tagatose 1,6-bisphosphate to D-glyceraldehyde 3-phosphate plus dihydroxyacetone phosphate. Remarkably, this gene cluster was located in 93% of the completely sequenced genomes of the E. coli B2 phylogroup, which contains the majority of extraintestinal pathogenic and adherent-invasive E. coli strains prevalent in patients with inflammatory bowel disease. This highlights the importance of understanding the clinical significance of D-tagatose in microbiota alterations. © 2022 The Author(s)
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