A double layer agar plate method results in an improvement for enumerating Vibrio vulnificus and Vibrio parahaemolyticus exposed to nutrient deficiency and refrigeration temperature
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Jae-Hyun | - |
dc.contributor.author | Lee, Sun-Young | - |
dc.date.accessioned | 2023-02-19T17:40:20Z | - |
dc.date.available | 2023-02-19T17:40:20Z | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 0740-0020 | - |
dc.identifier.issn | 1095-9998 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/60758 | - |
dc.description.abstract | This study sought to І)investigate the effect of varying concentrations of salt on the survival of Gram-negative bacteria in artificial seawater (ASW; pH 6) stored at 4 °C, ІІ)examine the culturable populations of Vibrio vulnificus persisting in ASW with or without 1000 U/mg catalase or 0.1% sodium pyruvate (pH 6) under aerobic and oxygen-restricted environments at 4 °C, and ІІІ)determine whether double layer agar plate (DLAP) methods can improve the culturability of V. vulnificus and Vibrio parahaemolyticus in ASW (pH 6) stored at 4 °C. Once incubated in ASW containing 10% salt at 4 °C, Escherichia coli O157:H7, Salmonella Typhimurium, and Shigella flexneri survived at levels over at least 3.42–4.65 log CFU/mL on day 28, but V. vulnificus underwent the inability to produce colonies within 7 days, while maintaining its membrane integrity. A positive correlation between sodium pyruvate and maintenance of platable colony-forming capability was observed in V. vulnificus incubated aerobically. Using tryptic soy agar containing 3% salt (TSAS) overlayed by thiosulfate-citrate-bile salts-sucrose agar (TCBS), a higher platable colony count of Vibrio spp. was recovered from ASW after 25 or 30 days of storage at 4 °C, followed by TSAS, TSAS overlayed by marine agar (MA), MA, and TCBS. © 2022 Elsevier Ltd | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | A double layer agar plate method results in an improvement for enumerating Vibrio vulnificus and Vibrio parahaemolyticus exposed to nutrient deficiency and refrigeration temperature | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fm.2022.104085 | - |
dc.identifier.bibliographicCitation | Food Microbiology, v.107 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000821317200002 | - |
dc.identifier.scopusid | 2-s2.0-85132928996 | - |
dc.citation.title | Food Microbiology | - |
dc.citation.volume | 107 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Double layer agar plate method | - |
dc.subject.keywordAuthor | Nutrient deficiency | - |
dc.subject.keywordAuthor | Salt | - |
dc.subject.keywordAuthor | Sodium private | - |
dc.subject.keywordAuthor | Viable but nonculturable | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RESUSCITATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.