A double layer agar plate method results in an improvement for enumerating Vibrio vulnificus and Vibrio parahaemolyticus exposed to nutrient deficiency and refrigeration temperature
- Authors
- Yoon, Jae-Hyun; Lee, Sun-Young
- Issue Date
- Oct-2022
- Publisher
- Academic Press
- Keywords
- Double layer agar plate method; Nutrient deficiency; Salt; Sodium private; Viable but nonculturable
- Citation
- Food Microbiology, v.107
- Journal Title
- Food Microbiology
- Volume
- 107
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/60758
- DOI
- 10.1016/j.fm.2022.104085
- ISSN
- 0740-0020
1095-9998
- Abstract
- This study sought to І)investigate the effect of varying concentrations of salt on the survival of Gram-negative bacteria in artificial seawater (ASW; pH 6) stored at 4 °C, ІІ)examine the culturable populations of Vibrio vulnificus persisting in ASW with or without 1000 U/mg catalase or 0.1% sodium pyruvate (pH 6) under aerobic and oxygen-restricted environments at 4 °C, and ІІІ)determine whether double layer agar plate (DLAP) methods can improve the culturability of V. vulnificus and Vibrio parahaemolyticus in ASW (pH 6) stored at 4 °C. Once incubated in ASW containing 10% salt at 4 °C, Escherichia coli O157:H7, Salmonella Typhimurium, and Shigella flexneri survived at levels over at least 3.42–4.65 log CFU/mL on day 28, but V. vulnificus underwent the inability to produce colonies within 7 days, while maintaining its membrane integrity. A positive correlation between sodium pyruvate and maintenance of platable colony-forming capability was observed in V. vulnificus incubated aerobically. Using tryptic soy agar containing 3% salt (TSAS) overlayed by thiosulfate-citrate-bile salts-sucrose agar (TCBS), a higher platable colony count of Vibrio spp. was recovered from ASW after 25 or 30 days of storage at 4 °C, followed by TSAS, TSAS overlayed by marine agar (MA), MA, and TCBS. © 2022 Elsevier Ltd
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