Raman Thermometry Nanopipettes in Cancer Photothermal Therapy
- Authors
- Ngo, Dinh Nghi; Ho, Vuong Thi Thanh Xuan; Kim, Gun; Song, Min Seok; Kim, Mi Ri; Choo, Jaebum; Joo, Sang-Wo; Lee, So Yeong
- Issue Date
- May-2022
- Publisher
- American Chemical Society
- Citation
- Analytical Chemistry, v.94, no.17, pp 6463 - 6472
- Pages
- 10
- Journal Title
- Analytical Chemistry
- Volume
- 94
- Number
- 17
- Start Page
- 6463
- End Page
- 6472
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/61390
- DOI
- 10.1021/acs.analchem.1c04452
- ISSN
- 0003-2700
1520-6882
- Abstract
- Raman thermometry based on surface-enhanced Raman scattering has been developed using nanopipettes in cancer cell photothermal therapy (PTT). Gold nanorods (AuNRs) are robustly epoxied on glass pipettes with a high surface coverage of 95% and less than 10 nm-wide nanogaps for intracellular thermometry and photothermal cancer therapy. The temperature changes could be estimated from the NC band shifts of 4-fluorophenyl isocyanide (FPNC)-adsorbed AuNRs on the Raman thermometry nanopipette (RTN) surfaces. An intracellular temperature change of 2.7 °C produced by altering the [Ca2+] in A431 cells was detected using the RTN in vitro, as checked from fura-2 acetoxymethyl ester (fura-2 AM) fluorescence images. For in vivo experiments, local temperature rises of 9.2 °C were observed in the mouse skin, whereas infrared camera images could not tract due to spatial resolution. In addition, a tumor growth suppression was observed in the PTT processes after an administration of the three AuNR-coated nanopipettes combined with a 671 nm laser irradiation for 5 min in 30 days. These results demonstrate not only the localized temperature sensing ability of FPNC-tagged AuNR nanopipettes in cell biology but also anti-cancer effects in photothermal cancer therapy. © 2021 American Chemical Society. All rights reserved.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.