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Expansion of the prime editing modality with Cas9 from Francisella novicidaopen access
- Authors
- Oh,Yeounsun; Lee, Wi‑jae; Hur, Junho K; Song, Woo Jeung; Lee, Youngjeon; Kim, Hanseop; Gwon, Lee Wha; Kim, Young‑Hyun; Park, Young‑Ho; Kim, Chan Hyoung; Lim, Kyung‑Seob; Song, Bong‑Seok; Huh, Jae‑Won; Kim, Sun‑Uk; Jun, Bong‑Hyun; Jung, Cheulhee; Lee, Seung Hwan
- Issue Date
- Apr-2022
- Publisher
- BioMed Central Ltd
- Keywords
- CRISPR-Cas9; Francisella novicida; Ortholog; Prime editing; Target expansion
- Citation
- Genome Biology, v.23, no.1
- Journal Title
- Genome Biology
- Volume
- 23
- Number
- 1
- ISSN
- 1474-7596
- Abstract
- Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines. © 2022, The Author(s).
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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