Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice
- Authors
- Chen, Sidong; Yoo, Hanna; Li, Chun Hei; Park, Chungwon; Park, Gyunam; Tan, Li Yang; Jung, Sangyong; Park, Hyokeun
- Issue Date
- 22-Oct-2021
- Publisher
- CELL PRESS
- Keywords
- Biological sciences; Neuroscience; Techniques in neuroscience
- Citation
- ISCIENCE, v.24, no.10
- Journal Title
- ISCIENCE
- Volume
- 24
- Number
- 10
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/62117
- DOI
- 10.1016/j.isci.2021.103181
- ISSN
- 2589-0042
2589-0042
- Abstract
- Although defective synaptic transmission was suggested to play a role in neurodegenerative diseases, the dynamics and vesicle viols of synaptic vesicles during neurodegeneration remain elusive. Here, we performed real-time three-dimensional tracking of single synaptic vesicles in cortical neurons from a mouse model of Huntington's disease (HD). Vesicles in HD neurons had a larger net displacement and radius of gyration compared with wild-type neurons. Vesicles with high release probability (P-r) were interspersed with low-P-r vesicles in HD neurons, whereas high-P-r vesicles were closer to fusion sites than low-P-r in wild-type neurons. Non-releasing vesicles in HD neurons had an abnormally high prevalence of irregular oscillatory motion. These abnormal dynamics and vesicle pools were rescued by overexpressing Rab11, and the abnormal irregular oscillatory motion was rescued by jasplakinolide. Our studies reveal the abnormal dynamics and pools of synaptic vesicles in the early stages of HD, suggesting a possible pathogenic mechanism of neurodegenerative diseases.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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