Organophosphorus pesticides exert estrogen receptor agonistic effect determined using Organization for Economic Cooperation and Development PBTG455, and induce estrogen receptor-dependent adipogenesis of 3T3-L1 adipocytes
- Authors
- Kim, Jin Tae; Lee, Hong Jin; Lee, Hee-Seok
- Issue Date
- Aug-2021
- Publisher
- Elsevier Ltd
- Keywords
- Adipogenesis; Agonist; Estrogen receptor; Organophosphorus pesticides
- Citation
- Environmental Pollution, v.283
- Journal Title
- Environmental Pollution
- Volume
- 283
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/62224
- DOI
- 10.1016/j.envpol.2021.117090
- ISSN
- 0269-7491
1873-6424
- Abstract
- Various chemicals containing pesticides can induce adipogenesis and cause obesity. Organophosphorus pesticides have been used for pest control. Here, we investigated the estrogen receptor (ER)-dependent adipogenesis-inducing effect of representative organophosphorus pesticides (OPs), diazinon, phoxim, terbufos and tolclofos-methyl in 3T3-L1 adipocytes. Four OPs exhibited ER agonistic effect, determined using the OECD Performance Based Test Guideline No. 455; in vitro ER stably transfected transactivation assay using ERα-HeLa-9903 cell line, through binding affinity to ERα. Additionally, they increased lipid droplet accumulation in a dose-dependent manner, which was suppressed by ICI182,780, a well-known ER antagonist. Four OPs treatment induced peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and perilipin expression. Furthermore, PPARγ, C/EBPα and perilipin expression was inhibited by co-treatment with ICI182,780. The increased mRNA expression of lipoprotein lipase and fatty acid synthase by four OPs was suppressed by co-treatment with ICI182,780. These results indicated that diazinon, phoxim, terbufos, and tolclofos-methyl might have adipogenesis-inducing effect mediated by interacting with ER. © 2021 Elsevier Ltd
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