Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments
- Authors
- Jo, Kyungae; Kim, Singeun; Yu, Kwang-Won; Chung, Young Bae; Kim, Woo Jung; Suh, Hyung Joo; Kim, Hoon
- Issue Date
- May-2022
- Publisher
- WILEY
- Keywords
- Dendrobium officinale; physicochemical pretreatment; polysaccharide; immunostimulating activity; glucomannan
- Citation
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.102, no.7, pp 3021 - 3028
- Pages
- 8
- Journal Title
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
- Volume
- 102
- Number
- 7
- Start Page
- 3021
- End Page
- 3028
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/62718
- DOI
- 10.1002/jsfa.11642
- ISSN
- 0022-5142
1097-0010
- Abstract
- BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kg(f) (7.5DO and 9DO). Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes). RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP. However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS. The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less. In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide. CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation. (c) 2021 Society of Chemical Industry
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Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
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