Efficient methods for isolating five phytochemicals from Gentiana macrophylla using high-performance countercurrent chromatography
DC Field | Value | Language |
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dc.contributor.author | Rho, Taewoong | - |
dc.contributor.author | Jung, Mila | - |
dc.contributor.author | Lee, Min Won | - |
dc.contributor.author | Chin, Young-Won | - |
dc.contributor.author | Yoon, Kee Dong | - |
dc.date.available | 2019-03-08T11:59:44Z | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 1615-9306 | - |
dc.identifier.issn | 1615-9314 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6420 | - |
dc.description.abstract | Efficient high-performance countercurrent chromatography methods were developed to isolate five typical compounds from the extracts of Gentiana macrophylla. n-Butanol-soluble extract of G. macrophylla contained three hydrophilic iridoids, loganic acid (1), swertiamarin (2) and gentiopicroside (3), and a chromene derivative, macrophylloside D (4) which were successfully isolated by flow rate gradient (1.5 mL/min in 0-60 min, 5.0 mL/min in 60-120 min), and consecutive flow rate gradient HPCCC using n-butanol/0.1% aqueous trifluoroacetic acid (1:1, v/v, normal phase mode) system. The yields of 1-4 were 22, 16, 122, and 6 mg, respectively, with purities over 97% in a flow rate gradient high-performance countercurrent chromatography, and consecutive flow rate gradient high-performance countercurrent chromatography gave 1, 2, 3 (54, 41, 348 mg, respectively, purities over 97%) and 4 (13 mg, purity at 95%) from 750 mg of sample. The main compound in methylene chloride soluble extract, 2-methoxyanofinic acid, was successfully separated by n-hexane/ethyl acetate/methanol/water (4:6:4:6, v/v/v/v, flow-rate: 4 mL/min, reversed phase mode) condition. The structures of five isolates were elucidated by H-1, C-13 NMR and ESI-Q-TOF-MS spectroscopic data which were compared with previously reported values. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Efficient methods for isolating five phytochemicals from Gentiana macrophylla using high-performance countercurrent chromatography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jssc.201600988 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SEPARATION SCIENCE, v.39, no.24, pp 4723 - 4731 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000393030400006 | - |
dc.identifier.scopusid | 2-s2.0-85004009890 | - |
dc.citation.endPage | 4731 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 4723 | - |
dc.citation.title | JOURNAL OF SEPARATION SCIENCE | - |
dc.citation.volume | 39 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Chromens | - |
dc.subject.keywordAuthor | Gentiana macrophylla | - |
dc.subject.keywordAuthor | High-performance countercurrent chromatography | - |
dc.subject.keywordAuthor | Iridoids | - |
dc.subject.keywordPlus | SCALE-UP PROCESS | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | SCABRA | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ROOTS | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | MODEL | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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