Efficient methods for isolating five phytochemicals from Gentiana macrophylla using high-performance countercurrent chromatography
- Authors
- Rho, Taewoong; Jung, Mila; Lee, Min Won; Chin, Young-Won; Yoon, Kee Dong
- Issue Date
- Dec-2016
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Chromens; Gentiana macrophylla; High-performance countercurrent chromatography; Iridoids
- Citation
- JOURNAL OF SEPARATION SCIENCE, v.39, no.24, pp 4723 - 4731
- Pages
- 9
- Journal Title
- JOURNAL OF SEPARATION SCIENCE
- Volume
- 39
- Number
- 24
- Start Page
- 4723
- End Page
- 4731
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6420
- DOI
- 10.1002/jssc.201600988
- ISSN
- 1615-9306
1615-9314
- Abstract
- Efficient high-performance countercurrent chromatography methods were developed to isolate five typical compounds from the extracts of Gentiana macrophylla. n-Butanol-soluble extract of G. macrophylla contained three hydrophilic iridoids, loganic acid (1), swertiamarin (2) and gentiopicroside (3), and a chromene derivative, macrophylloside D (4) which were successfully isolated by flow rate gradient (1.5 mL/min in 0-60 min, 5.0 mL/min in 60-120 min), and consecutive flow rate gradient HPCCC using n-butanol/0.1% aqueous trifluoroacetic acid (1:1, v/v, normal phase mode) system. The yields of 1-4 were 22, 16, 122, and 6 mg, respectively, with purities over 97% in a flow rate gradient high-performance countercurrent chromatography, and consecutive flow rate gradient high-performance countercurrent chromatography gave 1, 2, 3 (54, 41, 348 mg, respectively, purities over 97%) and 4 (13 mg, purity at 95%) from 750 mg of sample. The main compound in methylene chloride soluble extract, 2-methoxyanofinic acid, was successfully separated by n-hexane/ethyl acetate/methanol/water (4:6:4:6, v/v/v/v, flow-rate: 4 mL/min, reversed phase mode) condition. The structures of five isolates were elucidated by H-1, C-13 NMR and ESI-Q-TOF-MS spectroscopic data which were compared with previously reported values.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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