Tropicimonas arenosa sp nova, isolated from marine sand

Abstract

A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterial strain, designated CAU 1062(T), was isolated from marine sand in Jeju island, Republic of Korea and its taxonomic position was investigated using a polyphasic approach. CAU 1062(T) grew optimally at 30 degrees C and pH 8 in the presence of 2% (w/v) NaCI. Phylogenetic analysis based on 16S rRNA gene sequences showed that CAU 1062(T) formed a distinct lineage within the genus Tropicimonas and was the most closely related to Tropicimonas sediminicola M97(T) (similarity 96.11%). The strain had ubiquinone-10 (Q-10) as the predominant respiratory quinone and C-18:1 omega 7c as the major cellular fatty acid. The polar lipid pattern of CAU 1062(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an aminolipid, six phospholipids and five lipids. The DNA G+C content was 65.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, CAU 1062(T) represents a novel species of the genus Tropicimonas, for which the name Tropicimonas arenosa sp. nov. is proposed. The type strain is CAU 1062(T) (=KCTC 52178(T)=NBRC 111995(T)).