Flavihumibacter sediminis sp nov., isolated from tidal flat sediment

Abstract

A yellow, Gram-staining-positive, aerobic, rod-shaped and non-motile bacterial strain, designated CJ663(T), was isolated from The Tidal flat sediment in Ganghwa-do, South Korea. Strain CJ663(T) grew optimally on R2A at 30 degrees C and pH 7.0 and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain CJ663(T) belonged to the genus Flavihumibacter within The family Chitinophagaceae and was most closely related to Flavihumibacter cheonanensis KACC 17467(T) (98.3% similarity), followed by Flavihumibacter solisilvae KACC 17917(T) (97.4 %). DNA-DNA relatedness levels of strain CJ663(T) were 42.9% to F. cheonanensis KACC 17467(T) and 48.6% to F. solisilvae KACC 17917(T). The major isoprenoid quinone was menaquinone 7 (MK-7). The predominant polar lipids were phosphatidylethanolamine, aminophospholipid and two unidentified lipids. The major cellular fatty acids of strain CJ663(T) were iso-C-15 (: 0), anteiso-C-15 : 0 and iso-C-17 : 0 3-OH.The G + C content of the genomic DNA was 47.7 mol%. On the basis of data from this polyphasic taxonomic study, strain CJ663(T) represents a novel species in the genus Flavihumibacter, for which name Flavihumibacter sediminis sp. nov. is proposed; The type strain is CJ663(T) (=KACC 18874(T) = JCM 31431(T)).