Simple screening method for double-strand DNA binders using hairpin DNA-modified magnetic beads
DC Field | Value | Language |
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dc.contributor.author | Jo, H. | - |
dc.contributor.author | Min, K. | - |
dc.contributor.author | Song, K.-M. | - |
dc.contributor.author | Ku, J.K. | - |
dc.contributor.author | Han, M.S. | - |
dc.contributor.author | Ban, C. | - |
dc.date.accessioned | 2023-03-08T22:53:40Z | - |
dc.date.available | 2023-03-08T22:53:40Z | - |
dc.date.issued | 2011-01-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.issn | 1229-5949 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65042 | - |
dc.description.abstract | We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature (Tm). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Simple screening method for double-strand DNA binders using hairpin DNA-modified magnetic beads | - |
dc.type | Article | - |
dc.identifier.doi | 10.5012/bkcs.2011.32.1.247 | - |
dc.identifier.bibliographicCitation | Bulletin of the Korean Chemical Society, v.32, no.1, pp 247 - 250 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-78951481721 | - |
dc.citation.endPage | 250 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 247 | - |
dc.citation.title | Bulletin of the Korean Chemical Society | - |
dc.citation.volume | 32 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | dsDNA binder | - |
dc.subject.keywordAuthor | Hairpin DNA | - |
dc.subject.keywordAuthor | HPLC | - |
dc.subject.keywordAuthor | Magnetic bead | - |
dc.subject.keywordAuthor | Screening | - |
dc.subject.keywordPlus | Anticancer drug | - |
dc.subject.keywordPlus | Binding materials | - |
dc.subject.keywordPlus | DNA-binding | - |
dc.subject.keywordPlus | Double strands | - |
dc.subject.keywordPlus | dsDNA binder | - |
dc.subject.keywordPlus | Hairpin DNA | - |
dc.subject.keywordPlus | High-throughput screening | - |
dc.subject.keywordPlus | HPLC | - |
dc.subject.keywordPlus | HPLC analysis | - |
dc.subject.keywordPlus | Magnetic beads | - |
dc.subject.keywordPlus | Magnetic particle | - |
dc.subject.keywordPlus | Melting temperatures | - |
dc.subject.keywordPlus | Relative binding | - |
dc.subject.keywordPlus | Reverse-phase column | - |
dc.subject.keywordPlus | Screening methods | - |
dc.subject.keywordPlus | Binders | - |
dc.subject.keywordPlus | Functional materials | - |
dc.subject.keywordPlus | Magnetic separation | - |
dc.subject.keywordPlus | Magnetism | - |
dc.subject.keywordPlus | DNA | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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