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Simple screening method for double-strand DNA binders using hairpin DNA-modified magnetic beads
- Issue Date
- 20-Jan-2011
- Keywords
- dsDNA binder; Hairpin DNA; HPLC; Magnetic bead; Screening
- Citation
- Bulletin of the Korean Chemical Society, v.32, no.1, pp 247 - 250
- Pages
- 4
- Journal Title
- Bulletin of the Korean Chemical Society
- Volume
- 32
- Number
- 1
- Start Page
- 247
- End Page
- 250
- ISSN
- 0253-2964
1229-5949
- Abstract
- We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature (Tm). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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