A new set of rDNA-NTS-based multiple integrative cassettes for the development of antibiotic-marker-free recombinant yeasts
- Authors
- Moon, Hye Yun; Lee, Dong Wook; Sim, Gyu Hun; Kim, Hong-Jin; Hwang, Jee Youn; Kwon, Mun-Gyeong; Kang, Bo-Kyu; Kim, Jong Man; Kang, Hyun Ah
- Issue Date
- Sep-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Recombinant yeast; Multiple integration; Ribosomal DNA non-transcribed spacer; Defective auxotrophic markers
- Citation
- JOURNAL OF BIOTECHNOLOGY, v.233, pp 190 - 199
- Pages
- 10
- Journal Title
- JOURNAL OF BIOTECHNOLOGY
- Volume
- 233
- Start Page
- 190
- End Page
- 199
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6521
- DOI
- 10.1016/j.jbiotec.2016.07.006
- ISSN
- 0168-1656
1873-4863
- Abstract
- The traditional yeast Saccharomyces cerevisiae has been widely used as a host system to produce recombinant proteins and metabolites of great commercial value. To engineer recombinant yeast that stably maintains expression cassettes without an antibiotic resistance gene, we developed new multiple integration cassettes by exploiting the non-transcribed spacer (NTS) of ribosomal DNA (rDNA) in combination with defective selection markers. The 5' and 3'-fragments of rDNA-NTS2 were used as flanking sequences for the expression cassettes carrying a set of URA3, LEU2, HIS3, and TRPI selection markers with truncated promoters of different lengths. The integration numbers of NTS-based expression cassettes, ranging from one to 30 copies, showed a proportional increase with the extent of decreased expression of the auxotrophic markers. The NTS-based cassettes were used to construct yeast strains expressing the capsid protein of red-spotted grouper necrosis virus (RG-NNVCP) in a copy number-dependent manner. Oral administration of the recombinant yeast, harboring 30 copies of the integrated RG-NNVCP cassettes, provoked efficient immune responses in mice. In contrast, for the NTS cassettes expressing a truncated 3-hydroxyl-3-methylglutaryl-CoA reductase, the integrant carrying only 4 copies was screened as the highest producer of squalene, showing a 150-fold increase compared to that of the wild-type strain. The multiple integrated cassettes were stably retained under prolonged nonselective conditions. Altogether, our results strongly support that rDNA-NTS integrative cassettes are useful tools to construct recombinant yeasts carrying optimal copies of a desired expression cassette without an antibiotic marker gene, which are suitable as oral vaccines or feed additives for animal and human consumption. (C) 2016 Elsevier B.V. All rights reserved.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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