UBE2C cell-free RNA in urine can discriminate between bladder cancer and hematuriaopen access
- Authors
- Kim, Won Tae; Jeong, Pildu; Yan, Chunri; Kim, Ye Hwan; Lee, Il-Seok; Kang, Ho-Won; Kim, Yong-June; Lee, Sang-Cheol; Kim, Sang Jin; Kim, Yong Tae; Moon, Sung-Kwon; Choi, Yung-Hyun; Kim, Isaac Yi; Yun, Seok Joong; Kim, Wun-Jae
- Issue Date
- Sep-2016
- Publisher
- IMPACT JOURNALS LLC
- Keywords
- biomarkers; RNA; urinary bladder neoplasms; urine
- Citation
- ONCOTARGET, v.7, no.36, pp 58193 - 58202
- Pages
- 10
- Journal Title
- ONCOTARGET
- Volume
- 7
- Number
- 36
- Start Page
- 58193
- End Page
- 58202
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6526
- DOI
- 10.18632/oncotarget.11277
- ISSN
- 1949-2553
1949-2553
- Abstract
- Background: There is growing interest in circulating nucleic acids as cancer detection biomarkers. Therefore, the aim of the present study was to identify a key urinary cell-free RNA marker that may assist in the diagnosis of BC. Results: Five cell-free RNAs were selected as candidate cell-free RNAs from tissue microarray data. An area under the curve (AUC) cut-off value of 0.7 in receiver operating characteristic (ROC) curve analysis identified four urinary cell-free RNAs for further analysis (CDC20, ESM1, UBE2C, and CA9; AUC = 0.716, 0.704, 0.721, and 0.702, respectively). Binary logistic regression analysis revealed that high expression of UBE2C was significantly associated with BC (OR, 1.754; CI, 1.147-2.682; p = 0.010). Analysis of UBE2C expression in urine samples from BC patients and hematuria controls yielded an AUC of 0.839, with a sensitivity of 82.5% and a specificity of 76.2%. UBE2C levels was significantly increased in G2 and G3 tumors compared to normal controls (p < 0.001, respectively). Materials and methods: Urine samples from 212 BC patients and 106 normal controls (64 healthy individuals and 42 with hematuria) were examined. The candidate cell-free RNAs identified from tissue microarrays derived from BC and normal control tissues was then measured in the urine samples. Conclusions: The levels of urinary UBE2C cell-free RNA were significantly higher in BC samples than in normal and hematuria control samples. The higher levels of urinary UBE2C cell-free RNA in BC might reflect high expression in BC tissues. Therefore, urinary UBE2C cell-free RNA may be a valuable diagnostic marker for BC.
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