A DNA - Gold nanoparticle-based colorimetric competition assay for the detection of cysteine
- Authors
- Lee, J.-S.; Ulmann, P.A.; Han, M.S.; Mirkin, C.A.
- Issue Date
- Feb-2008
- Citation
- Nano Letters, v.8, no.2, pp 529 - 533
- Pages
- 5
- Journal Title
- Nano Letters
- Volume
- 8
- Number
- 2
- Start Page
- 529
- End Page
- 533
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65347
- DOI
- 10.1021/nl0727563
- ISSN
- 1530-6984
1530-6992
- Abstract
- We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg 2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg 2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods. © 2008 American Chemical Society.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65347)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.