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Reconstruction of rabbit corneal layer composed of corneal fibroblasts and corneal epithelium on the lyophilized amniotic membrane

Authors
Ahn, JJang, IYoon, MYSeo, YKYoon, HHKin, JSong, KYang, EPark, J
Issue Date
Mar-2006
Publisher
HIGHER EDUCATION PRESS
Keywords
lyophilized amniotic membrane; corneal layer; corneal fibroblast; corneal epithelium
Citation
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES, v.22, no.2, pp 150 - 156
Pages
7
Journal Title
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
Volume
22
Number
2
Start Page
150
End Page
156
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65436
DOI
10.1016/S1005-9040(06)60065-2
ISSN
1005-9040
2210-3171
Abstract
Many researchers have employed the cryopreserved amniotic membrane( CAM) and corneal epithelial cells in the treatment of a severely damaged burned cornea, with corneal epithelial cells cultured on an amniotic membrane (AM). The lyophilized amniotic membrane( LAM) has a higher graft take and a longer shelf life; it is easier to store and safer because of gamma irradiation. Two Teflon rings(Ahn's supporter) were made for culturing the cells on the LAM, and were then used to support the LAM. To reconstruct a corneal layer composed of corneal fibroblasts and epithelium, the comeal fibroblasts were first cultivated on the stromal side of LAM for five days, followed by epithelial cells culture on the epithelial side, by using the air-liquid interface culture. The reconstructed comeal layer composed of corneal fibroblasts and corneal epithelial cells has a much healthier basal layer of corneal epithelium than the reconstructed comeal epithelium, which was got by using only corneal epithelial cells, and resembles the epithelium of normal corneas, without the horny layer. Thus, the reconstruction of the comeal layer by using a LAM is considered to be a good in vitro model, not only for its application in toxicological test kits, but also for transplantation in patients with a severely damaged cornea.
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