Sequential binding of SeqA to paired hemi-methylated GATC sequences mediates formation of higher order complexesopen access
- Authors
- Han, Joo Seok; Kang, Sukhyun; Lee, Ho; Kim, Hak Kyun; Hwang, Deog Su
- Issue Date
- Sep-2003
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.37, pp 34983 - 34989
- Pages
- 7
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Volume
- 278
- Number
- 37
- Start Page
- 34983
- End Page
- 34989
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65615
- DOI
- 10.1074/jbc.M304923200
- ISSN
- 0021-9258
1083-351X
- Abstract
- Preferential binding of the SeqA protein to hemi-methylated GATC sequences functions as a negative regulator for Escherichia coli initiation of chromosomal replication at oriC and is implicated in segregating replicated chromosomes for cell division. We demonstrate that sequential binding of one SeqA tetramer to a set of two hemi-methylated sites mediates formation of higher-order complexes. The absence of cross-binding to separate DNAs suggests that two monomers of a SeqA tetramer bind to two hemi-methylated sites on DNA. The interaction among SeqA proteins bound to at least six adjacent hemi-methylated sites induces aggregation of free proteins to bound proteins. Aggregation might be indicative of SeqA foci, which appear to track replication forks in vivo. Studies of the properties of SeqA binding will contribute to our understanding of the function of SeqA.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65615)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.