Trigger Factor Interacts with DnaA Protein to Stimulateits Interaction with DnaA Box
- Authors
- Lee, Yong Sun; Lee, June; Kim, Hak Kyun; Kang, Sukhyun; Han, Joo Seok; Kim, Jae Bum; Hwang, Deog Su
- Issue Date
- 2003
- Publisher
- 한국통합생물학회
- Keywords
- DnaA protein; Trigger factor; DnaA box; Chaperone
- Citation
- Animal Cells and Systems, v.7, no.1, pp 81 - 87
- Pages
- 7
- Journal Title
- Animal Cells and Systems
- Volume
- 7
- Number
- 1
- Start Page
- 81
- End Page
- 87
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65785
- DOI
- 10.1080/12265071.2003.9647687
- ISSN
- 1976-8354
2151-2485
- Abstract
- While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coli chromosomal DNA replication, we found a 52-kD sized protein which bound to DnaA protein in a salt-dependent manner. This protein was identified as trigger factor, a ribosome-associated peptidyl-prolyl-cis/trans isomerase with chaperone activity. Trigger factor was overproduced and purified to near homogeneity, and its effect on the function of DnaA protein was examined. Enhanced binding of DnaA protein to DnaA box with no apparent supershift in the gel-shift experiments suggested that trigger factor, by virtue of its chaperone activity, exerts a change on DnaA protein thus increasing its binding affinity for DnaA box.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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