인삼 분획물이 Galactosamine에 의하여 손상된 일차배양한 흰쥐의 간세포에 미치는 영향

Abstract

The anti-hepatotoxic activity of Panax ginseng was studied using galgctosamine (GalN)-induced cytotoxicity in primary cultured rat hepatocytes. Panax genseng was fractionated into dammarane glycosides and protein fractions. The dammarane glycosides was further fractionated into panaxadiol and panaxantriol glycosides fractions. The protein fraction was further fractionated into four groups according to the molecular weight;larger than 10,000 dalton, between 5,000 and 10,000 dalton, between 1,000 and 5,000 dalton and between 500 and 1,000 dalton. A significant lowering action on the elevated glutamicpyruvic transaminase (GPT) activity in the culture medium of hepatocytes treated with 1.5mM GalN was noticed with all four protein fractions studied at the concentration of both 50mcg/ml and 100mcg/ml. Howevers, the effect of dammarane glycosides fractions was not significant. It was noted that the addition of 100mcg/ml of protein fractions smaller than 5,000 dalton significantly enhanced the syntheses of protein fractions significantly enhanced protein synthesis at the concentration of 100mcg/ml in the damaged hepatocytes by treatment of 1.5mM GalN.