위ㆍ장관 및 담관계 상피세포의 Glutathione S-Transferase출현도에 관한 면역조직화학적 관찰Immunohistochemical Studies of Glutathione S-Transferase on the Epithelium of Gastrointestinal and Biliary System
- Authors
- 송계용; 김영훈; 김미경; 박용욱; 유재형; 박상철
- Issue Date
- Sep-1993
- Publisher
- 중앙대학교 의과대학 의과학연구소
- Keywords
- glutathione S-transferase; adenocarcinoma; gastrointestinal tract
- Citation
- 중앙의대지, v.18, no.3, pp 277 - 288
- Pages
- 12
- Journal Title
- 중앙의대지
- Volume
- 18
- Number
- 3
- Start Page
- 277
- End Page
- 288
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66643
- ISSN
- 0253-6250
- Abstract
- Immunohistochemical studies were done on the isoenzymes of glutathione S-transferase(GST) to oberve the distribution pattern and to find out the major detoxifying GST isoenzymes of the normal and cancer cells in the gastrointestinal and biliary system. Immunohistochemical staining for the acidic placental isoenzyme(GSP-Pi) and basic liver isoenzymes(GST-L) using polyclonal antibody raised in the rabbit were carried out by perxodiase antiperoxidase method.
The results obtained were as follows;
1. GST-Pi stained weakly and strongly in the normal surface epithelium of the gastrointestinal and biliary system, respectively and negative in mucous cells. Staining intensity of GST-L shows similiar with GST-Pi in normal gastrointestinal tract but much lower in biliary system.
2. GST-Pi enzyme is markedly increased in the adenocarcinoma cells compared to the normal cells in all gastrointestinal and biliary system. While negative in the signet ring cells type.
3. Major detoxyfying isoenzyme is the placental acidic glutathione S-transferase(GST-Pi) rather than other GST isoenzymes in the adenocarcinoma cells of the gastrointestinal and biliary system.
- Files in This Item
-
Go to Link
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.